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sodium acetate buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sodium acetate buffer
    Sodium Acetate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acetic+buffer/Acetate+Buffer/pm42365067-88-16-21
    Average 97 stars, based on 1 article reviews
    sodium acetate buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Evaluation of Selected Brain Damage Biomarkers in the Determination of Brain Damage in Dogs With Neurological Distemper
    Article Snippet: .. At the end of the reaction, the PCR products were transferred to an agarose gel (Vivantis, Malaysia) prepared with 2% 1× TAE (Tris‐Acetic Acid‐EDTA) buffer containing 0.01% gel red (Invitrogen SYBR Safe DNA Gel Stain) and electrophoresed (Serva, Blue Marine 100, Germany) at 100 V current (Serva Blue Power Plus, Germany) for 70 min. At the end of the process, the products were analysed in a gel imaging device using a UV transilluminator and gel documentation system and samples showing a band in the 640 bp region were considered positive for the presence of CDV. ..

    Agarose Gel Electrophoresis:

    Article Title: Evaluation of Selected Brain Damage Biomarkers in the Determination of Brain Damage in Dogs With Neurological Distemper
    Article Snippet: .. At the end of the reaction, the PCR products were transferred to an agarose gel (Vivantis, Malaysia) prepared with 2% 1× TAE (Tris‐Acetic Acid‐EDTA) buffer containing 0.01% gel red (Invitrogen SYBR Safe DNA Gel Stain) and electrophoresed (Serva, Blue Marine 100, Germany) at 100 V current (Serva Blue Power Plus, Germany) for 70 min. At the end of the process, the products were analysed in a gel imaging device using a UV transilluminator and gel documentation system and samples showing a band in the 640 bp region were considered positive for the presence of CDV. ..

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: Integrative taxonomy of a monopisthocotylan (Platyhelminthes, Neodermata) parasite from the gills of Doras higuchii Sabaj Pérez & Birindelli (Siluriformes, Doradidae) from the Xingú River Basin, Pará, Brazil.
    Article Snippet: .. The amplicons were subjected to electrophoresis on a 1.5% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with SYBRsafe (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) alongside a 1 kb Plus DNA Ladder (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) at 100 V for 30 min. .. The PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, USA) and sequencing was carried out using the BigDye® Terminator Cycle Sequencing Kit v.3.1 Kit (Applied BiosystemsTM) on an ABI 3730 automatic sequencer (Applied Biosystems, California, USA) from the company OMIKKA (São Paulo State, Brazil), with the same primers used to amplify the 28S rDNA fragment, plus primers 900F, 1200R (Littlewood & Olson, 2001).

    Staining:

    Article Title: Evaluation of Selected Brain Damage Biomarkers in the Determination of Brain Damage in Dogs With Neurological Distemper
    Article Snippet: .. At the end of the reaction, the PCR products were transferred to an agarose gel (Vivantis, Malaysia) prepared with 2% 1× TAE (Tris‐Acetic Acid‐EDTA) buffer containing 0.01% gel red (Invitrogen SYBR Safe DNA Gel Stain) and electrophoresed (Serva, Blue Marine 100, Germany) at 100 V current (Serva Blue Power Plus, Germany) for 70 min. At the end of the process, the products were analysed in a gel imaging device using a UV transilluminator and gel documentation system and samples showing a band in the 640 bp region were considered positive for the presence of CDV. ..

    Article Title: Specificity and recognition of the ADP-ribosyl-ubiquitin modification in the DNA damage response
    Article Snippet: Protein samples were quantified using Bradford (BioRad) and equal amounts of protein were boiled in 1× NuPAGE LDS sample buffer (Invitrogen) with 60 mM DTT (Sigma-Aldrich) and resolved on NuPAGE Novex 4%–12% bis-tris gels (Invitrogen) in 1× NuPAGE MOPS SDS Running Buffer (Invitrogen) at 150 V. Proteins were transferred onto nitrocellulose membranes (Bio-Rad) using Trans-Blot Turbo Transfer System (Bio-Rad). .. The membranes were stained with Ponceau S Staining Solution (Thermo Fisher Scientific) to check the transfer quality, rinsed with water, and blocked in 5% (w/v) nonfat dried milk in PBS buffer with 0.1% (v/v) Tween 20 (PBST) for 1 hour at room temperature. ..

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: Integrative taxonomy of a monopisthocotylan (Platyhelminthes, Neodermata) parasite from the gills of Doras higuchii Sabaj Pérez & Birindelli (Siluriformes, Doradidae) from the Xingú River Basin, Pará, Brazil.
    Article Snippet: .. The amplicons were subjected to electrophoresis on a 1.5% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with SYBRsafe (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) alongside a 1 kb Plus DNA Ladder (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) at 100 V for 30 min. .. The PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, USA) and sequencing was carried out using the BigDye® Terminator Cycle Sequencing Kit v.3.1 Kit (Applied BiosystemsTM) on an ABI 3730 automatic sequencer (Applied Biosystems, California, USA) from the company OMIKKA (São Paulo State, Brazil), with the same primers used to amplify the 28S rDNA fragment, plus primers 900F, 1200R (Littlewood & Olson, 2001).

    Article Title: A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.
    Article Snippet: .. Blotted membranes were incubated in Ponceau S staining solution 19 (Thermo Fisher Scientific) for 5 min, before excessive solution was washed away with 20 distilled H2O. ..

    Imaging:

    Article Title: Evaluation of Selected Brain Damage Biomarkers in the Determination of Brain Damage in Dogs With Neurological Distemper
    Article Snippet: .. At the end of the reaction, the PCR products were transferred to an agarose gel (Vivantis, Malaysia) prepared with 2% 1× TAE (Tris‐Acetic Acid‐EDTA) buffer containing 0.01% gel red (Invitrogen SYBR Safe DNA Gel Stain) and electrophoresed (Serva, Blue Marine 100, Germany) at 100 V current (Serva Blue Power Plus, Germany) for 70 min. At the end of the process, the products were analysed in a gel imaging device using a UV transilluminator and gel documentation system and samples showing a band in the 640 bp region were considered positive for the presence of CDV. ..

    High Performance Liquid Chromatography:

    Article Title: Kidney-Targeted Nanoparticle Delivery of Formoterol Mitigates Diabetic Kidney Disease without Adverse Cardiac Effects
    Article Snippet: Mitochondrial dysfunction plays a critical role in the progression of diabetic kidney disease (DKD).. Previous research indicates that the FDAapproved β2-adrenergic receptor agonist and bronchodilator formoterol are efficacious at restoring mitochondrial function and slowing DKD progression.. Unfortunately, β2-adrenergic receptor antagonists have been shown to result in cardiovascular toxicity.

    Electrophoresis:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: Integrative taxonomy of a monopisthocotylan (Platyhelminthes, Neodermata) parasite from the gills of Doras higuchii Sabaj Pérez & Birindelli (Siluriformes, Doradidae) from the Xingú River Basin, Pará, Brazil.
    Article Snippet: .. The amplicons were subjected to electrophoresis on a 1.5% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with SYBRsafe (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) alongside a 1 kb Plus DNA Ladder (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA) at 100 V for 30 min. .. The PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, USA) and sequencing was carried out using the BigDye® Terminator Cycle Sequencing Kit v.3.1 Kit (Applied BiosystemsTM) on an ABI 3730 automatic sequencer (Applied Biosystems, California, USA) from the company OMIKKA (São Paulo State, Brazil), with the same primers used to amplify the 28S rDNA fragment, plus primers 900F, 1200R (Littlewood & Olson, 2001).

    SYBR Green Assay:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Incubation:

    Article Title: A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.
    Article Snippet: .. Blotted membranes were incubated in Ponceau S staining solution 19 (Thermo Fisher Scientific) for 5 min, before excessive solution was washed away with 20 distilled H2O. ..

    other:

    Article Title: Taxane chemotherapy promotes response to TIM-3 checkpoint blockade via STING-mediated ER stress and HMGB1 secretion.
    Article Snippet: To minimize non-specific staining, the cells were then incubated in a 1:2000 dilution of Fc block (TruStain FcX PLUS [anti-mouse CD16/32], Biolegend #156604).

    Article Title: Unravelling the bi-functional role of cation-regulated surface hydroxyl kinetics in Pt-catalyzed alcohol fuel cells.
    Article Snippet: Commercial carbon supported Pt catalyst (Pt/C, 20 wt% Pt nanoparticles on carbon black), nafion solution (5 wt.%) and perchloric acid (HClO4, 48~50%) were bought from Alfa Aesar.



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